recombinant human gdf-5  (PeproTech)

Journal: JCI Insight

Article Title: Impaired 1,25-dihydroxyvitamin D 3 action underlies enthesopathy development in the Hyp mouse model of X-linked hypophosphatemia

doi: 10.1172/jci.insight.163259

Figure Lengend Snippet: ( A ) Primary murine chondrocytes were pretreated for 0.5, 1, 4, or 18 hours with or without (–) 1 × 10 –8 M 1,25D prior to incubation with (+) or without (–) 100 ng/mL rhGDF5 (for 30 minutes) and subjected to Western blot analyses for p-SMAD1/5/9, SMAD1, and β-actin. Data are representative of those obtained from 3 independent experiments. ( B ) Primary murine chondrocytes were pretreated for 18 hours with 1 × 10 –8 M 1,25D followed by treatment with 200 ng/mL rhGDF5 (for 4 hours). Gene expression analyses were performed for BMP and IHH target genes. Data are representative of those obtained from 5–7 independent experiments. One-way ANOVA followed by Fisher’s least significant difference test was used to analyze significance between all genotype groups. * P < 0.05 versus WT; # P < 0.05 versus rhGDF5; a indicates P < 0.05 versus rhGDF5 plus 1,25D. ( C ) IHC for VDR was performed on P7, P14, P30, and P60 entheses from WT, Hyp , and C –/– mice. In all representative pictures, the enthesis region is outlined with a black box. Scale bar: 20 μm. Data are representative of 6 mice per age or genotype group.

Article Snippet: For Western blot analyses, chondrocytes were pretreated for 0.5, 1, 4, or 18 hours with 1 × 10 –8 M 1,25D prior to exposure to recombinant human GDF5 (rhGDF5) (100 ng/mL; R&D Systems) for 30 minutes.

Techniques: Incubation, Western Blot, Expressing

Journal: iScience

Article Title: Time- and cell-specific activation of BMP signaling restrains chondrocyte hypertrophy

doi: 10.1016/j.isci.2024.110537

Figure Lengend Snippet:

Article Snippet: Recombinant GDF-5 , Peprotech , Cat. No. 120-01.

Techniques: Recombinant, Plasmid Preparation, RNA Sequencing Assay, Software